-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria
نویسندگان
چکیده
منابع مشابه
beta-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other gram-negative bacteria.
A series of transposons are described which contain the gusA gene, encoding beta-glucuronidase (GUS), expressed from a variety of promoters, both regulated and constitutive. The regulated promoters include the tac promoter which can be induced by IPTG, and nifH promoters which are symbiotically activated in legume nodules. One transposon contains gusA with a strong Shine-Dalgarno translation in...
متن کاملGenetic tools for pseudomonads, rhizobia, and other gram-negative bacteria.
Gram-negative bacteria are extraordinarily diverse microorganisms that present a wide variety of characteristics worthy of genetic investigation. For historical reasons, the application of recombinant DNA technology to gram-negative bacteria in general has always lagged behind that of E. coli and its close relatives. However, the past 10 years have seen dramatic advances in the development of n...
متن کاملDiversity of retron elements in a population of rhizobia and other gram-negative bacteria.
Genetic elements called retrons reside on the chromosome of Escherichia coli and the myxobacteria and represent the first reverse transcriptase-encoding element to be found in a prokaryotic cell. All known retrons produce a functionally obscure RNA-DNA satellite molecule called multicopy single-stranded DNA (msDNA). We report here the presence of msDNA-producing retron elements in a number of n...
متن کاملAntibacterial Activity Studies of Salvia Mirzayanii and Salvia Atropatana against Six Standard Gram Positive and Gram Negative Bacteria
In this study antimicrobial effects of Salivia mirzayanii and Saliva atropatana were evaluated against six gram-positive and gram-negative bacteria by immersion bioautography, cylinder plate and tube dilution methods separately. In bioautography method, essential oils of Salvia mirzayanii and Salvia atropatana were separated on silicagel TLC plates by toluene-ethyl acetate (93-7). In cylander p...
متن کاملpostnatal studies of bats (pipistrellus kuhlii and miniopterus schreibersii) & histomorphology and histochemistry studies of organs and diseases of (neurergus microspilotus and n. kaiseri)
1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology
سال: 1995
ISSN: 1350-0872,1465-2080
DOI: 10.1099/13500872-141-7-1691